In 2021 Medical Technologies Congress (TIPTEKNO), Antalya, 2021
Low-Cost top stage incubator for live cell imaging applications
Zeynep Ozbilgin, Sevdenur Aksit, M. Fatih Toy
Atıf
@inproceedings{ozbilgin2021low,
title={Low-cost top stage incubator for live cell imaging applications},
author={{\"O}zbilgin, Zeynep and Toy, M Fatih and Ak{\c{s}}it, Sevdenur},
booktitle={2021 Medical Technologies Congress (TIPTEKNO)},
pages={1--4},
year={2021},
organization={IEEE}
}Özet
A top stage incubator is an essential equipment for long term imaging of live cells on a standard microscope. A low cost alternative to off-the-shelf top stage incubators is realized by using 3D printing technology. A closed system is designed for keeping the inner temperature at the desired value and an ITO coated glass plate is employed as a heater. The temperature inside the incubator is stabilized by using PID (Proportional-Integral-Derivative) algorithm that is controlled via Arduino Uno microcontroller. It takes approximately 20 minutes to stabilize the temperature at the desired value. The peak to peak temperature fluctuation after stabilization is ±1°C. Thus, the portable, user-friendly, easily accessible system allows long-term live cell imaging.
