Conference Paper

Here, an integrated portable system is presented that can maintain cell cultures at desired temperature in a 3D printed enclosure while imaging them in lens free inline holographic microscopy modality. Such a system is well suited for tissue culturing and monitoring at limited resources settings.

In this work we took an alternative route to these problems by combining maskless lithography with digital holography. Addition of digital holography enables the use of feedback by measuring the quantitative phase of specimen near real time and in situ nondestructively. After each near UV exposure, phase retardation map of exposed photopolymer is measured with digital holography part of the system. Any deviation from target phase is corrected by changing the pattern displayed on the mask.

A label free quantitative phase imaging method is realized in this project to enable imaging of an interface between different media. This system is based on an off-axis holographic microscope and uses a high numerical aperture (NA) microscope objective to achieve total internal reflection (TIR).

A low cost alternative to off-the-shelf top stage incubators is realized by using 3D printing technology. A closed system is designed for keeping the inner temperature at the desired value and an ITO coated glass plate is employed as a heater. The temperature inside the incubator is stabilized by using PID (Proportional-Integral-Derivative) algorithm that is controlled via Arduino Uno microcontroller.

We present an improved prism spectrometer with high wavelength range and spectral resolution.

Here it is proposed to implement an off-axis holography layout that utilizes a single wedge prism.

Refractive index tomography as an emerging technique enables the 3D morphological investigation of cells with no marker. Here, refractive index tomographic imaging of myelinating glial cells is presented.

Digital Holographic Microscopy (DHM) yields reconstructed complex wavefields. It allows synthesizing the aperture of a virtual microscope up to 2π, offering super-resolution phase images. Live images of micro-organisms and neurons with resolution less than 100 nm are presented.

As an illustration of the resolution improvement achieved with the synthetized aperture approach, Fig. 1 shows the resolution improvement obtained on a phase image. In summary, synthetizing the spatial frequencies spectrum in the reciprocal space leads to resolution improvement.

Experiments on live myoblast cells are carried out on two different platforms; random positioning machine (RPM), a ground base microgravity simulation platform, and parabolic flight campaign (PFC), a fixed wing plane flight providing short durations of alternating gravity conditions. Results show clear perinuclear phase increase. During seconds scale microgravity exposure, measurable scale morphological modifications are observed with the accumulated effect of repetitive exposures and short breaks.