Abstract
A top stage incubator is an essential equipment for long term imaging of live cells on a standard microscope. A low cost alternative to off-the-shelf top stage incubators is realized by using 3D printing technology. A closed system is designed for keeping the inner temperature at the desired value and an ITO coated glass plate is employed as a heater. The temperature inside the incubator is stabilized by using PID (Proportional-Integral-Derivative) algorithm that is controlled via Arduino Uno microcontroller. It takes approximately 20 minutes to stabilize the temperature at the desired value. The peak to peak temperature fluctuation after stabilization is ±1°C. Thus, the portable, user-friendly, easily accessible system allows long-term live cell imaging.
Zeynep Ozbilgin
Undergraduate Research Assistant
Sevdenur Aksit
Undergraduate Research Assistant
M. Fatih Toy
Associate Professor of Electrical and Electronics Engineering
My research interests include digital holography, quantitative phase imaging, optical diffraction tomography and optical superresolution.